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01
April
2022

Scientists finally decode the first complete human genome, which sat almost-complete for two decades

Scientists say they have finally assembled the full genetic blueprint for human life, adding the missing pieces to a puzzle nearly completed two decades ago.

Key points:

  • Scientists have deciphered the remaining roughly 8 per cent of the genome
  • The development opens the door for more medical discoveries
  • The research was published on Thursday in the journal Science

An international team described the first sequencing of a complete human genome — the set of instructions to build and sustain a human being — in research published this week in the journal Science.

The previous effort, celebrated across the world, was incomplete because DNA sequencing technologies of the day were not able to read certain parts of it.

Even after updates, it was missing about 8 per cent of the genome.

Solving the puzzle

“Some of the genes that make us uniquely human were actually in this ‘dark matter of the genome’ and they were totally missed,” said Evan Eichler, a University of Washington researcher who participated in the current effort and the original Human Genome Project.

“It took 20-plus years, but we finally got it done.”

Many — including Professor Eichler’s own students — thought it had been finished already.

“I was teaching them, and they said, ‘Wait a minute. Isn’t this like the sixth time you guys have declared victory?’ I said, ‘No, this time we really, really did it!”

Scientists said this full picture of the genome will give humanity a greater understanding of our evolution and biology while also opening the door to medical discoveries in areas like ageing, neurodegenerative conditions, cancer and heart disease.

“We’re just broadening our opportunities to understand human disease,” said Karen Miga, an author of one of the six studies published on Thursday.

The research caps off decades of work.

Work in progress

The first draft of the human genome was announced in a White House ceremony in 2000 by leaders of two competing entities: an international publicly funded project led by an agency of the US National Institutes of Health and a private company, Maryland-based Celera Genomics.

The human genome is made up of about 3.1 billion DNA sub-units, pairs of chemical bases known by the letters A, C, G and T.

Genome editing
The human genome is made up of about 3.1 billion DNA sub-units, in pairs.(iStock/Getty Images: Andy)

Genes are strings of these lettered pairs that contain instructions for making proteins, the building blocks of life.

Humans have about 30,000 genes, organised in 23 groups called chromosomes that are found in the nucleus of every cell.

Before now, there were “large and persistent gaps that have been in our map, and these gaps fall in pretty important regions,” Dr Miga said.

Dr Miga, a genomics researcher at the University of California-Santa Cruz, worked with Adam Phillippy of the National Human Genome Research Institute to organise the team of scientists to start from scratch with a new genome with the aim of sequencing all of it, including previously missing pieces.

The group, named after the sections at the very ends of chromosomes, called telomeres, is known as the Telomere-to-Telomere, or T2T, consortium.

Major breakthrough

Their work adds new genetic information to the human genome, corrects previous errors and reveals long stretches of DNA known to play important roles in both evolution and disease.

A version of the research was published last year before being reviewed by scientific peers.

“This is a major improvement, I would say, of the Human Genome Project,” doubling its impact, said geneticist Ting Wang of the Washington University School of Medicine in St Louis, who was not involved in the research.

Professor Eichler said some scientists used to think unknown areas contained “junk”.

Not him.

“Some of us always believed there was gold in those hills,” he said.

It turns out that gold includes many important genes, he said, such as ones integral to making a person’s brain bigger than a chimp’s, with more neurons and connections.

To find such genes, scientists needed new ways to read life’s cryptic genetic language.

Reading genes requires cutting the strands of DNA into pieces hundreds to thousands of letters long.

Sequencing machines read the letters in each piece and scientists try to put the pieces in the right order. That’s especially tough in areas where letters repeat.

Scientists said some areas were illegible before improvements in gene sequencing machines that now allow them to, for example, accurately read a million letters of DNA at a time.

That allows scientists to see genes with repeated areas as longer strings instead of snippets that they had to later piece together.

Researchers also had to overcome another challenge: Most cells contain genomes from both mother and father, confusing attempts to assemble the pieces correctly.

T2T researchers got around this by using a cell line from one “complete hydatidiform mole,” an abnormal fertilised egg containing no fetal tissue that has two copies of the father’s DNA and none of the mother’s. 

The future

The next step? Mapping more genomes, including ones that include collections of genes from both parents. 

This effort did not map one of the 23 chromosomes that is found in males, called the Y chromosome, because the mole contained only an X. 

Dr Wang said he was working with the T2T group on the Human Pangenome Reference Consortium, which is trying to generate “reference”, or template, genomes for 350 people representing the breadth of human diversity. 

“Now we’ve gotten one genome right and we have to do many, many more,” Professor Eichler said. 

“This is the beginning of something really fantastic for the field of human genetics.” 

Professor Eichler is paid by the Howard Hughes Medical Institute, which also supports The Associated Press’s health and science department. 

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